Proteomics

Location
Facility

Proteomics

Facility Contact

Rachael Protzman

Rachael Protzman

The SAHMRI Proteomics Core Facility was established in 2019, but its history goes back ten years further as it grew out of the mass spectrometry group of the Lysosomal Diseases Research Unit in Adelaide.

Our team has many years of proteomics experience with special interests in phosphoproteomics and plasma proteomics.

Services

The Facility operates on a fee-for-service basis to help with cost recovery, but our main aim is to help our clients to do great science.

The facility is newly equipped with a Bruker timsTOF Pro2 mass spectrometer, generously funded by the ACRF (Australian Cancer Research Foundation) as part of the ACRF Centre for Integrated Cancer Systems Biology. This latest generation mass spectrometer is ideally suited to label-free proteomics. It is exceptionally fast and can acquire over 120 MS/MS spectra per second, ideal for complex proteomics samples (MS/MS spectra provide amino acid sequence information on proteins).

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Equipment list

Equipment Type
Access
Bruker timsTOF Pro2 - AHMS
Access: Service

FAQs

You are very welcome to prepare your own samples for analysis. Samples will be run by facility staff at a reduced fee used primarily to cover instrument maintenance costs. Please contact facility staff to discuss sample preparation and clean-up methods.

The timsTOF Pro2 is an extremely sensitive instrument. The limiting factor is usually liquid handling in sample preparation rather than protein concentration. The correct amount of sample needs to be evaluated on a case-by-case basis. As a guide, for tryptic digests of cells 1 µL of 200ng/µL digest solution is usually the optimal amount of sample to load although results can be obtained from more dilute samples.

This varies with the annual funding cycle, but usually waiting times are less than two months.

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