Lipidomics and Metabolomics
The SAHMRI Metabolomics and Lipidomics Core Facility is focused on providing a world-class small molecule analysis service.
The facility offers a wide range of services ranging from global plasma lipidomics, metabolomics, fluxomics to highly targeted quantification of small molecules, both endogenous such as neurotransmitters and xenobiotic like small molecule drugs.
Although only established as a core facility in 2019, the group has a long history going back over two decades. It grew out of the mass spectrometry group of the Lysosomal Diseases Research Unit in Adelaide, then primarily working on mass spectrometry-based analysis methods to study the primary and secondary substrates of lysosomal storage disorders (lipids and glycosaminoglycans).
The facility operates on a fee-for-service basis. Some equipment can be booked subject to training and permission from the Facility Manager.
Services and Capabilities
- Metabolomics and Fluxomics using an Agilent 6546 QTof coupled to a 1290 Infinity II UHPLC system and Bruker timsTOF Flex
- Lipidomics using a Waters Xevo G2-XS QTof coupled to a Waters ACQUITY UPLC I-Class system
- Targeted LC-MS/MS using a SCIEX 4000 QTrap or SCIEX 5500 QqQ coupled to a Waters ACQUITY UPLC system
- Development of validated quantitative methods following US FDA Guidelines for Bioanalysis
- Automated sample preparation for large sample sets using Agilent Bravo Metabolomics Sample Prep Platform
- Data analysis
- Bruker MetaboScape
- Agilent VistaFlux
- Agilent MassHunter
- Waters Progenesis QI
- Skyline
- MetaboAnalyst
Equipment list
- College of Medicine and Public Health, Flinders University
- College of Science and Engineering, Flinders University
- Biochemistry and Molecular Biology, Monash University
FAQs
Mostly mass analyses are on a fee for service basis as the assays require high levels of training to perform. Under certain circumstances, for example exceptionally large numbers of samples or previous mass spectrometry experience, it may be possible to run your own samples. Please contact the facility’s manager to discuss.
Mass Spectrometry is a very versatile technique and many common sample types are amenable to analysis. These include, but are not limited to plasma, urine, CSF (cerebrospinal fluid), tissue homogenates, faeces, and dried blood spots.
A very wide range of lipids, metabolites, drugs, drug metabolites, and environmental pollutants important in human health are measurable using the technology available at SAHMRI. Please contact the friendly facility staff to discuss your specific requirements. You may also want to search the scientific literature including the search terms LC-MS (Liquid chromatography mass spectrometry) or LC-MS/MS.
Sponsors and partnerships
SAHMRI is located on Kaurna Country. We pay respects to the Kaurna people of the Adelaide Plains and to all Aboriginal and Torres Strait Islander people. We are committed to embracing knowledge and culture as we continue our working journey to incorporate Aboriginal health research across all of our themes and further reconciliation.
Read more"Tirki nguta tappa tangka marninithi"
(Learning and knowledge are the pathway to alter the mind)
- Uncle Lewis Yerloburka O’Brien, Kaurna Elder
